mmp inhibitor known col-3 Search Results


mmp inhibitor incyclinide  (MedChemExpress)
94
MedChemExpress mmp inhibitor incyclinide
(A) Primary human nasal epithelial cells (pooled from 14 human donors) were cultured at the air-liquid interface to mimic the human respiratory tract and challenged with SARS-CoV-2. Acetylated tubulin levels were determined by anti-AcTub immunofluorescence and DAPI was used to stain nuclei. 3D reconstructions of xy and yz fields are shown. (B) Cells were subjected to total cellular RNA extraction at 48 hours post inoculation with 10000 plaque forming units of WA1, BA.1, or BA.2, and RT-qPCR of viral ORF1a was performed. Relative ORF1a transcript abundance compared to actin was determined by the 2^(-ΔΔCT) method. Symbols represent independent RT-qPCR runs. (C) Infectious virus titers from cell culture medium were measured by challenging Vero cells followed by fixation and immunostaining with anti-N antibody. Infectious units were quantified by measuring focus forming units with high-content imaging. Symbols represent results from three independent infections of Vero cells. (D) RT-qPCR of cellular Ifnb was performed at 48 hours post inoculation. Relative Ifnb transcript abundance compared to actin was determined by the 2^(-ΔΔCT) method. Symbols represent independent RT-qPCR runs. (E) Recombinant WA.1 encoding mCherry and Spike protein from WA1, Delta, or BA.1 were used to inoculate nasal ALI and infection was measured at 24 hours by mCherry fluorescence. Representative fields of view are shown. Scale bars = 300 μm. (F) Summary mCherry fluorescence from three independent infections is shown. The fluorescence intensity of WA1 (WA1 Spike)-mCherry was set to 1. (G) Nasal ALI were pretreated with 10 μM E64d, 10 μM camostat mesylate, a combination of 10 μM E64d and 10 μM camostat mesylate, 10 μM <t>incyclinide,</t> or 1 μM bafilomycin A1 for 2 hours. Afterwards, cells were challenged with WA.1 or BA.1 at an MOI of 0.05. At 48 hours post inoculation, cells were subjected to total cellular RNA extraction and ORF1a levels were measured by RT-qPCR. Relative ORF1a transcript abundance compared to actin was determined by the 2^(-ΔΔCT) method. Symbols represent independent RT-qPCR runs. Results are represented as means plus standard error. Statistically significant differences (* P < 0.05) between the indicated condition and the corresponding data point of WA.1 were determined by one-way ANOVA. ns; not significant. NT; not tested (bafilomycin A1 was not tested against WA1). Cartoon made with Biorender.com .
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mmp inhibitor metastat  (MetaStat Inc)
90
MetaStat Inc mmp inhibitor metastat
(A) Primary human nasal epithelial cells (pooled from 14 human donors) were cultured at the air-liquid interface to mimic the human respiratory tract and challenged with SARS-CoV-2. Acetylated tubulin levels were determined by anti-AcTub immunofluorescence and DAPI was used to stain nuclei. 3D reconstructions of xy and yz fields are shown. (B) Cells were subjected to total cellular RNA extraction at 48 hours post inoculation with 10000 plaque forming units of WA1, BA.1, or BA.2, and RT-qPCR of viral ORF1a was performed. Relative ORF1a transcript abundance compared to actin was determined by the 2^(-ΔΔCT) method. Symbols represent independent RT-qPCR runs. (C) Infectious virus titers from cell culture medium were measured by challenging Vero cells followed by fixation and immunostaining with anti-N antibody. Infectious units were quantified by measuring focus forming units with high-content imaging. Symbols represent results from three independent infections of Vero cells. (D) RT-qPCR of cellular Ifnb was performed at 48 hours post inoculation. Relative Ifnb transcript abundance compared to actin was determined by the 2^(-ΔΔCT) method. Symbols represent independent RT-qPCR runs. (E) Recombinant WA.1 encoding mCherry and Spike protein from WA1, Delta, or BA.1 were used to inoculate nasal ALI and infection was measured at 24 hours by mCherry fluorescence. Representative fields of view are shown. Scale bars = 300 μm. (F) Summary mCherry fluorescence from three independent infections is shown. The fluorescence intensity of WA1 (WA1 Spike)-mCherry was set to 1. (G) Nasal ALI were pretreated with 10 μM E64d, 10 μM camostat mesylate, a combination of 10 μM E64d and 10 μM camostat mesylate, 10 μM <t>incyclinide,</t> or 1 μM bafilomycin A1 for 2 hours. Afterwards, cells were challenged with WA.1 or BA.1 at an MOI of 0.05. At 48 hours post inoculation, cells were subjected to total cellular RNA extraction and ORF1a levels were measured by RT-qPCR. Relative ORF1a transcript abundance compared to actin was determined by the 2^(-ΔΔCT) method. Symbols represent independent RT-qPCR runs. Results are represented as means plus standard error. Statistically significant differences (* P < 0.05) between the indicated condition and the corresponding data point of WA.1 were determined by one-way ANOVA. ns; not significant. NT; not tested (bafilomycin A1 was not tested against WA1). Cartoon made with Biorender.com .
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mmp inhibitors metastat  (MetaStat Inc)
90
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(A) Primary human nasal epithelial cells (pooled from 14 human donors) were cultured at the air-liquid interface to mimic the human respiratory tract and challenged with SARS-CoV-2. Acetylated tubulin levels were determined by anti-AcTub immunofluorescence and DAPI was used to stain nuclei. 3D reconstructions of xy and yz fields are shown. (B) Cells were subjected to total cellular RNA extraction at 48 hours post inoculation with 10000 plaque forming units of WA1, BA.1, or BA.2, and RT-qPCR of viral ORF1a was performed. Relative ORF1a transcript abundance compared to actin was determined by the 2^(-ΔΔCT) method. Symbols represent independent RT-qPCR runs. (C) Infectious virus titers from cell culture medium were measured by challenging Vero cells followed by fixation and immunostaining with anti-N antibody. Infectious units were quantified by measuring focus forming units with high-content imaging. Symbols represent results from three independent infections of Vero cells. (D) RT-qPCR of cellular Ifnb was performed at 48 hours post inoculation. Relative Ifnb transcript abundance compared to actin was determined by the 2^(-ΔΔCT) method. Symbols represent independent RT-qPCR runs. (E) Recombinant WA.1 encoding mCherry and Spike protein from WA1, Delta, or BA.1 were used to inoculate nasal ALI and infection was measured at 24 hours by mCherry fluorescence. Representative fields of view are shown. Scale bars = 300 μm. (F) Summary mCherry fluorescence from three independent infections is shown. The fluorescence intensity of WA1 (WA1 Spike)-mCherry was set to 1. (G) Nasal ALI were pretreated with 10 μM E64d, 10 μM camostat mesylate, a combination of 10 μM E64d and 10 μM camostat mesylate, 10 μM <t>incyclinide,</t> or 1 μM bafilomycin A1 for 2 hours. Afterwards, cells were challenged with WA.1 or BA.1 at an MOI of 0.05. At 48 hours post inoculation, cells were subjected to total cellular RNA extraction and ORF1a levels were measured by RT-qPCR. Relative ORF1a transcript abundance compared to actin was determined by the 2^(-ΔΔCT) method. Symbols represent independent RT-qPCR runs. Results are represented as means plus standard error. Statistically significant differences (* P < 0.05) between the indicated condition and the corresponding data point of WA.1 were determined by one-way ANOVA. ns; not significant. NT; not tested (bafilomycin A1 was not tested against WA1). Cartoon made with Biorender.com .
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col-3  (CollaGenex Pharmaceuticals)
90
CollaGenex Pharmaceuticals col-3
(A) Primary human nasal epithelial cells (pooled from 14 human donors) were cultured at the air-liquid interface to mimic the human respiratory tract and challenged with SARS-CoV-2. Acetylated tubulin levels were determined by anti-AcTub immunofluorescence and DAPI was used to stain nuclei. 3D reconstructions of xy and yz fields are shown. (B) Cells were subjected to total cellular RNA extraction at 48 hours post inoculation with 10000 plaque forming units of WA1, BA.1, or BA.2, and RT-qPCR of viral ORF1a was performed. Relative ORF1a transcript abundance compared to actin was determined by the 2^(-ΔΔCT) method. Symbols represent independent RT-qPCR runs. (C) Infectious virus titers from cell culture medium were measured by challenging Vero cells followed by fixation and immunostaining with anti-N antibody. Infectious units were quantified by measuring focus forming units with high-content imaging. Symbols represent results from three independent infections of Vero cells. (D) RT-qPCR of cellular Ifnb was performed at 48 hours post inoculation. Relative Ifnb transcript abundance compared to actin was determined by the 2^(-ΔΔCT) method. Symbols represent independent RT-qPCR runs. (E) Recombinant WA.1 encoding mCherry and Spike protein from WA1, Delta, or BA.1 were used to inoculate nasal ALI and infection was measured at 24 hours by mCherry fluorescence. Representative fields of view are shown. Scale bars = 300 μm. (F) Summary mCherry fluorescence from three independent infections is shown. The fluorescence intensity of WA1 (WA1 Spike)-mCherry was set to 1. (G) Nasal ALI were pretreated with 10 μM E64d, 10 μM camostat mesylate, a combination of 10 μM E64d and 10 μM camostat mesylate, 10 μM <t>incyclinide,</t> or 1 μM bafilomycin A1 for 2 hours. Afterwards, cells were challenged with WA.1 or BA.1 at an MOI of 0.05. At 48 hours post inoculation, cells were subjected to total cellular RNA extraction and ORF1a levels were measured by RT-qPCR. Relative ORF1a transcript abundance compared to actin was determined by the 2^(-ΔΔCT) method. Symbols represent independent RT-qPCR runs. Results are represented as means plus standard error. Statistically significant differences (* P < 0.05) between the indicated condition and the corresponding data point of WA.1 were determined by one-way ANOVA. ns; not significant. NT; not tested (bafilomycin A1 was not tested against WA1). Cartoon made with Biorender.com .
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prinomastat (ag3340)  (MetaStat Inc)
90
MetaStat Inc prinomastat (ag3340)
(A) Primary human nasal epithelial cells (pooled from 14 human donors) were cultured at the air-liquid interface to mimic the human respiratory tract and challenged with SARS-CoV-2. Acetylated tubulin levels were determined by anti-AcTub immunofluorescence and DAPI was used to stain nuclei. 3D reconstructions of xy and yz fields are shown. (B) Cells were subjected to total cellular RNA extraction at 48 hours post inoculation with 10000 plaque forming units of WA1, BA.1, or BA.2, and RT-qPCR of viral ORF1a was performed. Relative ORF1a transcript abundance compared to actin was determined by the 2^(-ΔΔCT) method. Symbols represent independent RT-qPCR runs. (C) Infectious virus titers from cell culture medium were measured by challenging Vero cells followed by fixation and immunostaining with anti-N antibody. Infectious units were quantified by measuring focus forming units with high-content imaging. Symbols represent results from three independent infections of Vero cells. (D) RT-qPCR of cellular Ifnb was performed at 48 hours post inoculation. Relative Ifnb transcript abundance compared to actin was determined by the 2^(-ΔΔCT) method. Symbols represent independent RT-qPCR runs. (E) Recombinant WA.1 encoding mCherry and Spike protein from WA1, Delta, or BA.1 were used to inoculate nasal ALI and infection was measured at 24 hours by mCherry fluorescence. Representative fields of view are shown. Scale bars = 300 μm. (F) Summary mCherry fluorescence from three independent infections is shown. The fluorescence intensity of WA1 (WA1 Spike)-mCherry was set to 1. (G) Nasal ALI were pretreated with 10 μM E64d, 10 μM camostat mesylate, a combination of 10 μM E64d and 10 μM camostat mesylate, 10 μM <t>incyclinide,</t> or 1 μM bafilomycin A1 for 2 hours. Afterwards, cells were challenged with WA.1 or BA.1 at an MOI of 0.05. At 48 hours post inoculation, cells were subjected to total cellular RNA extraction and ORF1a levels were measured by RT-qPCR. Relative ORF1a transcript abundance compared to actin was determined by the 2^(-ΔΔCT) method. Symbols represent independent RT-qPCR runs. Results are represented as means plus standard error. Statistically significant differences (* P < 0.05) between the indicated condition and the corresponding data point of WA.1 were determined by one-way ANOVA. ns; not significant. NT; not tested (bafilomycin A1 was not tested against WA1). Cartoon made with Biorender.com .
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mmp inhibitor cmt-3  (CollaGenex Pharmaceuticals)
90
CollaGenex Pharmaceuticals mmp inhibitor cmt-3
(A) Primary human nasal epithelial cells (pooled from 14 human donors) were cultured at the air-liquid interface to mimic the human respiratory tract and challenged with SARS-CoV-2. Acetylated tubulin levels were determined by anti-AcTub immunofluorescence and DAPI was used to stain nuclei. 3D reconstructions of xy and yz fields are shown. (B) Cells were subjected to total cellular RNA extraction at 48 hours post inoculation with 10000 plaque forming units of WA1, BA.1, or BA.2, and RT-qPCR of viral ORF1a was performed. Relative ORF1a transcript abundance compared to actin was determined by the 2^(-ΔΔCT) method. Symbols represent independent RT-qPCR runs. (C) Infectious virus titers from cell culture medium were measured by challenging Vero cells followed by fixation and immunostaining with anti-N antibody. Infectious units were quantified by measuring focus forming units with high-content imaging. Symbols represent results from three independent infections of Vero cells. (D) RT-qPCR of cellular Ifnb was performed at 48 hours post inoculation. Relative Ifnb transcript abundance compared to actin was determined by the 2^(-ΔΔCT) method. Symbols represent independent RT-qPCR runs. (E) Recombinant WA.1 encoding mCherry and Spike protein from WA1, Delta, or BA.1 were used to inoculate nasal ALI and infection was measured at 24 hours by mCherry fluorescence. Representative fields of view are shown. Scale bars = 300 μm. (F) Summary mCherry fluorescence from three independent infections is shown. The fluorescence intensity of WA1 (WA1 Spike)-mCherry was set to 1. (G) Nasal ALI were pretreated with 10 μM E64d, 10 μM camostat mesylate, a combination of 10 μM E64d and 10 μM camostat mesylate, 10 μM <t>incyclinide,</t> or 1 μM bafilomycin A1 for 2 hours. Afterwards, cells were challenged with WA.1 or BA.1 at an MOI of 0.05. At 48 hours post inoculation, cells were subjected to total cellular RNA extraction and ORF1a levels were measured by RT-qPCR. Relative ORF1a transcript abundance compared to actin was determined by the 2^(-ΔΔCT) method. Symbols represent independent RT-qPCR runs. Results are represented as means plus standard error. Statistically significant differences (* P < 0.05) between the indicated condition and the corresponding data point of WA.1 were determined by one-way ANOVA. ns; not significant. NT; not tested (bafilomycin A1 was not tested against WA1). Cartoon made with Biorender.com .
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mmp inhibitor metastat {col-3  (MetaStat Inc)
90
MetaStat Inc mmp inhibitor metastat {col-3
(A) Primary human nasal epithelial cells (pooled from 14 human donors) were cultured at the air-liquid interface to mimic the human respiratory tract and challenged with SARS-CoV-2. Acetylated tubulin levels were determined by anti-AcTub immunofluorescence and DAPI was used to stain nuclei. 3D reconstructions of xy and yz fields are shown. (B) Cells were subjected to total cellular RNA extraction at 48 hours post inoculation with 10000 plaque forming units of WA1, BA.1, or BA.2, and RT-qPCR of viral ORF1a was performed. Relative ORF1a transcript abundance compared to actin was determined by the 2^(-ΔΔCT) method. Symbols represent independent RT-qPCR runs. (C) Infectious virus titers from cell culture medium were measured by challenging Vero cells followed by fixation and immunostaining with anti-N antibody. Infectious units were quantified by measuring focus forming units with high-content imaging. Symbols represent results from three independent infections of Vero cells. (D) RT-qPCR of cellular Ifnb was performed at 48 hours post inoculation. Relative Ifnb transcript abundance compared to actin was determined by the 2^(-ΔΔCT) method. Symbols represent independent RT-qPCR runs. (E) Recombinant WA.1 encoding mCherry and Spike protein from WA1, Delta, or BA.1 were used to inoculate nasal ALI and infection was measured at 24 hours by mCherry fluorescence. Representative fields of view are shown. Scale bars = 300 μm. (F) Summary mCherry fluorescence from three independent infections is shown. The fluorescence intensity of WA1 (WA1 Spike)-mCherry was set to 1. (G) Nasal ALI were pretreated with 10 μM E64d, 10 μM camostat mesylate, a combination of 10 μM E64d and 10 μM camostat mesylate, 10 μM <t>incyclinide,</t> or 1 μM bafilomycin A1 for 2 hours. Afterwards, cells were challenged with WA.1 or BA.1 at an MOI of 0.05. At 48 hours post inoculation, cells were subjected to total cellular RNA extraction and ORF1a levels were measured by RT-qPCR. Relative ORF1a transcript abundance compared to actin was determined by the 2^(-ΔΔCT) method. Symbols represent independent RT-qPCR runs. Results are represented as means plus standard error. Statistically significant differences (* P < 0.05) between the indicated condition and the corresponding data point of WA.1 were determined by one-way ANOVA. ns; not significant. NT; not tested (bafilomycin A1 was not tested against WA1). Cartoon made with Biorender.com .
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Image Search Results


(A) Primary human nasal epithelial cells (pooled from 14 human donors) were cultured at the air-liquid interface to mimic the human respiratory tract and challenged with SARS-CoV-2. Acetylated tubulin levels were determined by anti-AcTub immunofluorescence and DAPI was used to stain nuclei. 3D reconstructions of xy and yz fields are shown. (B) Cells were subjected to total cellular RNA extraction at 48 hours post inoculation with 10000 plaque forming units of WA1, BA.1, or BA.2, and RT-qPCR of viral ORF1a was performed. Relative ORF1a transcript abundance compared to actin was determined by the 2^(-ΔΔCT) method. Symbols represent independent RT-qPCR runs. (C) Infectious virus titers from cell culture medium were measured by challenging Vero cells followed by fixation and immunostaining with anti-N antibody. Infectious units were quantified by measuring focus forming units with high-content imaging. Symbols represent results from three independent infections of Vero cells. (D) RT-qPCR of cellular Ifnb was performed at 48 hours post inoculation. Relative Ifnb transcript abundance compared to actin was determined by the 2^(-ΔΔCT) method. Symbols represent independent RT-qPCR runs. (E) Recombinant WA.1 encoding mCherry and Spike protein from WA1, Delta, or BA.1 were used to inoculate nasal ALI and infection was measured at 24 hours by mCherry fluorescence. Representative fields of view are shown. Scale bars = 300 μm. (F) Summary mCherry fluorescence from three independent infections is shown. The fluorescence intensity of WA1 (WA1 Spike)-mCherry was set to 1. (G) Nasal ALI were pretreated with 10 μM E64d, 10 μM camostat mesylate, a combination of 10 μM E64d and 10 μM camostat mesylate, 10 μM incyclinide, or 1 μM bafilomycin A1 for 2 hours. Afterwards, cells were challenged with WA.1 or BA.1 at an MOI of 0.05. At 48 hours post inoculation, cells were subjected to total cellular RNA extraction and ORF1a levels were measured by RT-qPCR. Relative ORF1a transcript abundance compared to actin was determined by the 2^(-ΔΔCT) method. Symbols represent independent RT-qPCR runs. Results are represented as means plus standard error. Statistically significant differences (* P < 0.05) between the indicated condition and the corresponding data point of WA.1 were determined by one-way ANOVA. ns; not significant. NT; not tested (bafilomycin A1 was not tested against WA1). Cartoon made with Biorender.com .

Journal: bioRxiv

Article Title: Omicron Spike confers enhanced infectivity and interferon resistance to SARS-CoV-2 in human nasal tissue

doi: 10.1101/2023.05.06.539698

Figure Lengend Snippet: (A) Primary human nasal epithelial cells (pooled from 14 human donors) were cultured at the air-liquid interface to mimic the human respiratory tract and challenged with SARS-CoV-2. Acetylated tubulin levels were determined by anti-AcTub immunofluorescence and DAPI was used to stain nuclei. 3D reconstructions of xy and yz fields are shown. (B) Cells were subjected to total cellular RNA extraction at 48 hours post inoculation with 10000 plaque forming units of WA1, BA.1, or BA.2, and RT-qPCR of viral ORF1a was performed. Relative ORF1a transcript abundance compared to actin was determined by the 2^(-ΔΔCT) method. Symbols represent independent RT-qPCR runs. (C) Infectious virus titers from cell culture medium were measured by challenging Vero cells followed by fixation and immunostaining with anti-N antibody. Infectious units were quantified by measuring focus forming units with high-content imaging. Symbols represent results from three independent infections of Vero cells. (D) RT-qPCR of cellular Ifnb was performed at 48 hours post inoculation. Relative Ifnb transcript abundance compared to actin was determined by the 2^(-ΔΔCT) method. Symbols represent independent RT-qPCR runs. (E) Recombinant WA.1 encoding mCherry and Spike protein from WA1, Delta, or BA.1 were used to inoculate nasal ALI and infection was measured at 24 hours by mCherry fluorescence. Representative fields of view are shown. Scale bars = 300 μm. (F) Summary mCherry fluorescence from three independent infections is shown. The fluorescence intensity of WA1 (WA1 Spike)-mCherry was set to 1. (G) Nasal ALI were pretreated with 10 μM E64d, 10 μM camostat mesylate, a combination of 10 μM E64d and 10 μM camostat mesylate, 10 μM incyclinide, or 1 μM bafilomycin A1 for 2 hours. Afterwards, cells were challenged with WA.1 or BA.1 at an MOI of 0.05. At 48 hours post inoculation, cells were subjected to total cellular RNA extraction and ORF1a levels were measured by RT-qPCR. Relative ORF1a transcript abundance compared to actin was determined by the 2^(-ΔΔCT) method. Symbols represent independent RT-qPCR runs. Results are represented as means plus standard error. Statistically significant differences (* P < 0.05) between the indicated condition and the corresponding data point of WA.1 were determined by one-way ANOVA. ns; not significant. NT; not tested (bafilomycin A1 was not tested against WA1). Cartoon made with Biorender.com .

Article Snippet: The MMP inhibitor incyclinide (HY-13648) was obtained from MedChemExpress.

Techniques: Cell Culture, Immunofluorescence, Staining, RNA Extraction, Quantitative RT-PCR, Virus, Immunostaining, Imaging, Recombinant, Infection, Fluorescence